A few weeks ago, I wrote a critical analysis of a paper by Christopher Shaw and Lucija Tomljenovic published in Inorganic Biochemistry that tried to convince us that aluminum adjuvants in vaccines caused autism. Predictably, it’s another anti-vaccine paper retracted by another journal.
Shaw and Tomljenovic have quite a history in retracted anti-vaccine articles. Last year, they wrote an article about aluminum adjuvants in the HPV vaccine – it was retracted, and eventually republished almost word-for-word in an inferior journal.
The paper by Shaw and co-workers is being retracted jointly by the authors and the editor.
Yup, another anti-vaccine paper retracted. Of course, the article was roundly criticized in numerous posts across the skeptical universe soon after it was published. For example, the piercing Orac wrote in his blog,
given Shaw and Tomljenovic’s history, it is not unreasonable to be suspicious of this study as well…
At best, what we have here are researchers with little or no expertise in very basic molecular biology techniques using old methodology that isn’t very accurate overinterpreting the differences in gene and protein levels that they found. At worst, what we have are antivaccine “researchers” who are not out for scientific accuracy but who actually want to promote the idea that vaccines cause autism….If this were a first offense, I’d give Shaw and Tomljenovic the benefit of the doubt, but this is far from their first offense.
Orac called the paper, “antivaccine pseudoscience.” Not that anti-vaccine is anything but pseudoscience.
Other bloggers, like The Mad Virologist, who is also an expert on DNA analysis, and the Blood Brain Barrier Scientist, who writes about the blood-brain barrier and heavy metal music, jointly analyzed the paper by Shaw and Tomljenovic. They did a masterful job in looking at some of the technical errors and other issues with the paper. I won’t pretend to summarize what they write, so I’ll use their own summary:
Based on the methods that were used in this paper, Shaw et al. went too far in declaring that aluminum adjuvants cause autism. But there are six other key points that limit what conclusions can be drawn from this paper:1) They selected genes based on old literature and ignored newer publications.2) The method for PCR quantification is imprecise and cannot be used as an absolute quantification of expression of the selected genes.3) They used inappropriate statistical tests that are more prone to giving significant results which is possibly why they were selected.4) Their dosing regime for the mice makes assumptions on the development of mice that are not correct.5) They gave the mice far more aluminum sooner than the vaccine schedule exposes children to.6) There are irregularities in both the semi-quantitative RT-PCR and Western blot data that strongly suggests that these images were fabricated. This is probably the most damning thing about the paper. If the data were manipulated and images fabricated, then the paper needs to be retracted and UBC needs to do an investigation into research misconduct by the Shaw lab.
Taken together, we cannot trust Shaw’s work here and if we were the people funding this work, we’d be incredibly ticked off because they just threw away money that could have done some good but was instead wasted frivolously. Maybe there’s a benign explanation for the irregularities that we’ve observed, but until these concerns are addressed this paper cannot be trusted.
Also, a lot of criticism occurred on PubPeer, a website devoted to a kind of extended peer review of published papers. The commenters were harsh. Oneclaimed that there was a “clear and deliberate” removal of control results in the paper. And many others either hinted or outright stated that DNA gel bands were duplicated, photoshopped or taken from a 2014 paper by Shaw and Tomljenovic (and published in a journal that’s not even indexed on PubMed).
Added to all of this is that the primary author has left the University of British Columbia, where Shaw and Tomljenovic do their “research,” taking all of the data with her. I don’t mean to be cynical, but I will be – yeah right.
As an aside, I don’t know why the University of British Columbia continues to house Shaw and Tomljenovic. They have had their “research” utterly discredited by the World Health Organization. They have had papers retracted. And when they do publish, they’re in low ranked, low impact factor journals. Finally, their “research” is supported by some of the most profoundly anti-vaccine sponsors in the world. Frankly, the only good Shaw and Tomljenovic do for the anti-vaccine world is to allow those of us who are pro-science/pro-vaccine lots of fodder in embarrassing the anti-vaccine world.
So there we go. Another Shaw and Tomljenovic anti-vaccine paper retracted, and will probably be republished in the Journal of Homeopathy Vaccines (no, it doesn’t exist). By the way, I won the betting pool on when it would be retracted. Hopefully, the shills pay me for winning.
- Inbar R, Weiss R, Tomljenovic L, Arango MT, Deri Y, Shaw CA, Chapman J, Blank M, Shoenfeld Y. WITHDRAWN: Behavioral abnormalities in young female mice following administration of aluminum adjuvants and the human papillomavirus (HPV) vaccine Gardasil. Vaccine. 2016 Jan 9. pii: S0264-410X(16)00016-5. doi: 10.1016/j.vaccine.2015.12.067. [Epub ahead of print] PubMed PMID: 26778424.
- Li D, Tomljenovic L, Li Y, Shaw C. Subcutaneous injections of aluminum at vaccine adjuvant levels activate innate immune genes in mouse brain that are homologous with biomarkers of autism. J Inorg Biochem. 2017 Dec;177:39-54. doi: 10.1016/j.jinorgbio.2017.08.035.